Lyophilization is the process of dehydrating a product after getting frozen. Lyophilization involves changing of ice directly into vapor without going in liquid condition. This technique has existed thousands of years ago for preserving food products for a very long time. With the modernization in techniques, the process of lyophilization has evolved vastly in the present day and time but the basics remain the same. Lyophilization is also commonly known as freeze-drying. This technique is also vastly used in the health and medicine industry. One of the main known application of lyophilization is the production of anti-rabies vaccine which has helped save millions of life. The vaccine is prepared by freeze drying of live rabies virus.